- Purity ≥95% by SDS-PAGE and HPLC; per-lot COA on request
- Certificate of analysis tied to your lot number
- Tracked EU delivery, dispatched the next working day
GDF-8 / Myostatin Propeptide
Recombinant myostatin propeptide — GDF-8's own latency domain
In stock1 format available
€123
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Ask about this listing or request its COA- FormLyophilized recombinant protein in a sealed glass vial
- Research areasMuscle-mass regulation, myostatin-pathway inhibition, cachexia and dystrophy models, bone and metabolic endpoints
For laboratory research use only. Sold exclusively for in-vitro laboratory research by qualified professionals. Not for human or veterinary use, and not a medicine, food, cosmetic or dietary supplement. Terms and Conditions
Overview
Key facts
Cells make myostatin as a longer precursor, which is then cut in two: the mature growth factor — a C-terminal dimer — and an N-terminal prodomain called the propeptide.
- Purity
- ≥95% by SDS-PAGE and HPLC; lot-matched COA available
- Form
- Lyophilized recombinant protein in a sealed glass vial
- Storage
- −20 °C, sealed and protected from light and moisture
- Available size
- 1 mg
For research use only. Not for human consumption.
Cells make myostatin as a longer precursor, which is then cut in two: the mature growth factor — a C-terminal dimer — and an N-terminal prodomain called the propeptide. Rather than simply falling away, the propeptide stays attached to the mature dimer, holding it in a latent complex that cannot activate its receptors until BMP-1/tolloid proteases release it. Adding the propeptide on its own is therefore the most natural way to switch off myostatin signalling in an experimental model — which is why it is used as a point of comparison for engineered ligand traps like ACE-031 and for follistatin.
The signalling route it interrupts is well understood. The mature ligand — two 109-residue chains linked by a disulfide — binds the type II receptors ActRIIB and ActRIIA, recruits ALK4 and ALK5 as type I receptors and signals by phosphorylating Smad2/3. As the propeptide acts on the ligand and not on the receptor, other ligands of ActRIIB are unaffected — a difference in selectivity that makes it a useful parallel arm next to ACE-031, which captures a far broader range. Each vial holds 1 mg of freeze-dried recombinant protein with purity of 95% or more by SDS-PAGE and HPLC; dissolve it gently in sterile or bacteriostatic water, add a carrier protein to dilute stocks and portion it out before freezing instead of thawing it repeatedly.
Specifications
Supply & purity
- Form
- Lyophilized recombinant protein in a sealed glass vial
- Purity
- ≥95% by SDS-PAGE and HPLC; lot-matched COA available
- Available sizes
- 1 mg
- Solubility
- Sterile or bacteriostatic water; carrier protein recommended for dilute stocks
Handling & storage
- Storage (lyophilized)
- −20 °C, sealed and protected from light and moisture
- Storage (reconstituted)
- 2–8 °C for immediate work; aliquot and freeze for longer studies
Additional detail
- Protein class
- TGF-beta superfamily prodomain (latency-associated propeptide)
- Gene
- MSTN, encoding growth differentiation factor 8
- Also known as
- GDF-8 propeptide, myostatin prodomain, latency-associated peptide of myostatin
- Mature ligand it binds
- Myostatin (GDF-8), a disulfide-linked homodimer of 109-residue subunits
- Receptor axis
- ActRIIB / ActRIIA with type I receptors ALK4 and ALK5, signalling through Smad2/3
- Latency release
- Cleavage of the propeptide by BMP-1/tolloid metalloproteinases frees the active dimer
- Research areas
- Muscle-mass regulation, myostatin-pathway inhibition, cachexia and dystrophy models, bone and metabolic endpoints
Questions about GDF-8 / Myostatin Propeptide
How does GDF-8 differ from the myostatin propeptide?
GDF-8 is myostatin itself — the active dimer that does the signalling. The propeptide is the N-terminal piece cut from the same precursor; it remains bound to the active dimer and keeps it switched off. Researchers therefore use it as the ligand's built-in inhibitor, not as the ligand.
Where does it sit relative to ACE-031 and Follistatin-344?
All three shut down the same pathway, each at its own point. ACE-031 is a lab-built, soluble version of the ActRIIB receptor that soaks up many ligands; follistatin is a naturally occurring protein that binds activins and myostatin; the propeptide is the part of myostatin that normally keeps it latent. Because their selectivity differs, studies often run them as parallel arms.
Is the material independently tested?
Yes. An independent lab analyses each lot, measuring purity by SDS-PAGE and HPLC and verifying identity with suitable protein methods. The certificate carries the lot number on your vial and can be requested before or after you order.
What are the steps for reconstitution and storage?
Let sterile or bacteriostatic water run slowly down the glass and swirl gently — don't vortex. Use the solution from the fridge (2–8 °C) for work that follows straight away, or divide it into portions and freeze them for longer projects. Unopened freeze-dried vials belong in the freezer (−20 °C), shielded from light and moisture.
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